Abstract
CYP4A11, the major fatty acid omega-hydroxylase in human liver is involved in the balance of lipids, but its role and regulation are both poorly understood. We studied the effects of retinoids on the regulation of CYP4A11 in the human hepatoma cell line HepaRG. Treatment of HepaRG cells with all-trans-retinoic acid resulted in a strong decrease in CYP4A11 gene expression and apoprotein content and, furthermore, was associated with a 50% decrease in the microsomal lauric acid hydroxylation activity. Such a strong suppression of CYP4A11 expression by retinoids could have a major impact on fatty acid metabolism in the liver.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carcinoma, Hepatocellular / enzymology
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Carcinoma, Hepatocellular / pathology
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Cell Line, Tumor
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Chromatography, High Pressure Liquid
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Cytochrome P-450 CYP4A
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Cytochrome P-450 Enzyme Inhibitors*
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Cytochrome P-450 Enzyme System / genetics
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Dose-Response Relationship, Drug
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Humans
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Liver / drug effects*
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Liver / enzymology
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Liver Neoplasms / enzymology
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Liver Neoplasms / pathology
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PPAR alpha / metabolism
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RNA, Messenger / genetics
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Tretinoin / pharmacology*
Substances
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Cytochrome P-450 Enzyme Inhibitors
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PPAR alpha
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RNA, Messenger
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Tretinoin
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Cytochrome P-450 Enzyme System
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CYP4A11 protein, human
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Cytochrome P-450 CYP4A