Abstract
In this study, we investigated whether orotate phosphoribosyl transferase (OPRT) correlates with the clinicopathological features and effect of 5-fluorouracil (5-FU) in human oral carcinoma. We examined the expression of OPRT mRNA by in situ hybridization in surgical specimens of oral squamous cell carcinoma. The expression of OPRT mRNA in oral carcinoma was observed in all specimens and such expression was higher than that seen in normal control tissue specimens. There was no correlation between the expression of OPRT mRNA and clinical factors, but the expression of OPRT mRNA was significantly associated with histological differentiation. The expression of OPRT mRNA showed correlation with effect of 5-FU for oral carcinoma in either in vivo or in vitro. These results suggest that the OPRT expressions may therefore be a prognostic factor of 5-FU efficacy in patients with oral squamous cell carcinoma.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Antineoplastic Agents / therapeutic use
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Carcinoma, Squamous Cell / drug therapy
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Carcinoma, Squamous Cell / enzymology*
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Carcinoma, Squamous Cell / pathology
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Case-Control Studies
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Chi-Square Distribution
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Dihydrouracil Dehydrogenase (NADP) / analysis
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Dihydrouracil Dehydrogenase (NADP) / genetics*
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Enzyme Activation
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Female
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Fluorouracil / therapeutic use
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Gene Expression
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Humans
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Immunohistochemistry
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In Situ Hybridization / methods
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Male
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Middle Aged
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Mouth Neoplasms / drug therapy
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Mouth Neoplasms / enzymology*
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Mouth Neoplasms / pathology
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Neoplasm Staging
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Orotate Phosphoribosyltransferase / analysis
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Orotate Phosphoribosyltransferase / genetics*
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RNA, Messenger / analysis*
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Regression Analysis
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Reverse Transcriptase Polymerase Chain Reaction
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Tumor Cells, Cultured
Substances
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Antineoplastic Agents
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RNA, Messenger
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Dihydrouracil Dehydrogenase (NADP)
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Orotate Phosphoribosyltransferase
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Fluorouracil