Abstract
The reaction responsible for replication error correction by mismatch repair proceeds via several steps: mismatch recognition, mismatch-provoked excision, repair DNA synthesis, and ligation. Key steps in this process are the recognition and subsequent exonucleolytic removal of the mispair. A minimal system comprised of human MutSalpha (MSH2*MSH6), MutLalpha (MLH1*PMS2), exonuclease I (EXOI), replication protein A (RPA), proliferating cell nuclear antigen (PCNA), and replication factor C (RFC) is sufficient to support mismatch-provoked excision in vitro. This chapter describes methods for analysis of the reconstituted excision reaction.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Base Pair Mismatch
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DNA Damage
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DNA Mismatch Repair*
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DNA Repair Enzymes
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DNA-Binding Proteins / metabolism
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Exodeoxyribonucleases / metabolism
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Humans
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MutL Proteins
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MutS Homolog 2 Protein / metabolism
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Neoplasm Proteins / metabolism
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Proliferating Cell Nuclear Antigen / metabolism
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Replication Protein A / metabolism
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Replication Protein C / metabolism
Substances
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DNA-Binding Proteins
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G-T mismatch-binding protein
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MutLalpha protein, human
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Neoplasm Proteins
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Proliferating Cell Nuclear Antigen
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Replication Protein A
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Exodeoxyribonucleases
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exodeoxyribonuclease I
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MSH2 protein, human
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MutL Proteins
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MutS Homolog 2 Protein
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Replication Protein C
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DNA Repair Enzymes