Abstract
Expression of the epidermal growth factor (EGF) receptor was evaluated by immunohistochemical staining of formalin-fixed, paraffin-embedded tumour tissues employing two antibodies raised to short synthetic peptides from the cytoplasmic domain of the molecule. Both antibodies gave concordant staining of a series of bladder cancers known to express or lack EGF receptors. There was no cross-reaction with the related c-erbB-2 protein, which was also over-expressed in some cases. Cancers with EGF receptor expression also expressed high levels of TGF-alpha, a receptor agonist.
MeSH terms
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Antibodies, Monoclonal
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ErbB Receptors / analysis*
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ErbB Receptors / genetics
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ErbB Receptors / immunology
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Gene Expression / physiology
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Humans
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Immunohistochemistry
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Paraffin
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Proto-Oncogene Proteins / analysis
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Proto-Oncogenes
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Receptor, ErbB-2
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Transforming Growth Factor alpha / analysis
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Transforming Growth Factor alpha / genetics
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Transforming Growth Factor alpha / immunology
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Urinary Bladder Neoplasms / chemistry*
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Urinary Bladder Neoplasms / genetics
Substances
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Antibodies, Monoclonal
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Proto-Oncogene Proteins
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Transforming Growth Factor alpha
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Paraffin
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ErbB Receptors
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Receptor, ErbB-2