Abstract
Early growth response 1 (Egr-1) is a cellular transcription factor involved in diverse biologic functions. Egr-1 has been associated with Epstein-Barr virus (EBV) infection, but it is still unknown whether any EBV protein regulates Egr-1 expression. In this study, we first showed that EBV reactivation is involved in upregulation of Egr-1 and that Egr-1 can be induced by Zta, an EBV lytic transactivator. Zta not only binds to the Egr-1 promoter but also activates the ERK signaling pathway to trigger binding of Elk-1 to the Egr-1 promoter. In addition, knockdown of Egr-1 significantly reduces the spontaneous expression of Zta and Rta in EBV-infected 293 cells, suggesting that a positive-feedback network involving Egr-1 is required for EBV reactivation. This study also implies that Zta has the potential to affect expression of certain genes through Egr-1.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Base Sequence
-
Cell Line
-
DNA-Binding Proteins / physiology*
-
Early Growth Response Protein 1 / genetics
-
Early Growth Response Protein 1 / physiology*
-
Epstein-Barr Virus Infections / genetics
-
Epstein-Barr Virus Infections / virology
-
Extracellular Signal-Regulated MAP Kinases / metabolism
-
Gene Expression Regulation, Viral*
-
Herpesvirus 4, Human / physiology*
-
Humans
-
Immediate-Early Proteins / physiology
-
Kidney / metabolism
-
Molecular Sequence Data
-
Promoter Regions, Genetic*
-
Protein Binding
-
Signal Transduction
-
Trans-Activators / physiology*
-
Transcription, Genetic
-
Transcriptional Activation
-
Viral Proteins / physiology*
-
ets-Domain Protein Elk-1 / genetics
-
ets-Domain Protein Elk-1 / metabolism
Substances
-
BRLF1 protein, Human herpesvirus 4
-
BZLF1 protein, Herpesvirus 4, Human
-
DNA-Binding Proteins
-
EGR1 protein, human
-
Early Growth Response Protein 1
-
Immediate-Early Proteins
-
Trans-Activators
-
Viral Proteins
-
ets-Domain Protein Elk-1
-
Extracellular Signal-Regulated MAP Kinases