Abstract
To study anti-inflammatory cytokine effects on RANKL+-T-cell-mediated osteoclastogenesis in vivo, we injected human interleukin-10 (hIL-10) into pathogen-infected HuPBL-NOD/SCID mice. The results show significantly decreased RANKL+ Th1-associated alveolar bone loss and coexpression of human gamma interferon (hIFN-gamma) and human macrophage colony-stimulating factor, but not hIL-4, in RANKL+ Th cells compatible with those from successfully treated aggressive periodontitis subjects. Thus, there are critical cytokine interactions linking hIFN-gamma+ Th1 cells to RANKL-RANK/OPG signaling for periodontal osteoclastogenesis in vivo.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Actinobacillus Infections / immunology
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Actinobacillus Infections / microbiology
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Actinobacillus Infections / physiopathology
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Adolescent
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Adult
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Aggregatibacter actinomycetemcomitans / immunology
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Aggregatibacter actinomycetemcomitans / pathogenicity*
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Alveolar Bone Loss / immunology*
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Alveolar Bone Loss / physiopathology
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Animals
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CD4 Antigens / metabolism*
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Carrier Proteins / metabolism*
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Humans
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Interferon-gamma / metabolism
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Interleukin-10 / pharmacology*
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Membrane Glycoproteins / metabolism*
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Mice
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Mice, Inbred NOD
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Mice, SCID
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Periodontitis / immunology
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Periodontitis / microbiology
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Periodontitis / physiopathology
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RANK Ligand
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Receptor Activator of Nuclear Factor-kappa B
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Th1 Cells / immunology
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Th1 Cells / metabolism*
Substances
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CD4 Antigens
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Carrier Proteins
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Membrane Glycoproteins
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RANK Ligand
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Receptor Activator of Nuclear Factor-kappa B
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TNFRSF11A protein, human
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TNFSF11 protein, human
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Tnfrsf11a protein, mouse
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Tnfsf11 protein, mouse
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Interleukin-10
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Interferon-gamma