Abstract
YidC is a member of the OxaI family of membrane proteins that has been implicated in the membrane insertion of inner membrane proteins in Escherichia coli. We have recently demonstrated that proteoliposomes containing only YidC support both the stable membrane insertion and the oligomerization of the c subunit of the F(1)F(0) ATP synthase (F(0)c). Here we have shown that two mutants of F(0)c unable to form a functional F(1)F(0) ATPase interact with YidC, require YidC for membrane insertion, but fail to oligomerize. These data show that oligomerization is not essential for the stable YidC-dependent membrane insertion of F(0)c consistent with a function of YidC as a membrane protein insertase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Substitution / genetics*
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Bacterial Proton-Translocating ATPases / chemistry
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Bacterial Proton-Translocating ATPases / genetics
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Bacterial Proton-Translocating ATPases / physiology*
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Escherichia coli / enzymology*
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Escherichia coli / genetics*
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Escherichia coli Proteins / chemistry
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / physiology*
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Intracellular Membranes / enzymology*
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Membrane Transport Proteins / chemistry
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Membrane Transport Proteins / physiology*
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Protein Processing, Post-Translational / genetics
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Protein Subunits / chemistry
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Protein Subunits / genetics
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Protein Subunits / metabolism
Substances
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Escherichia coli Proteins
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Membrane Transport Proteins
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Protein Subunits
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YIDC protein, E coli
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atpE protein, E coli
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Bacterial Proton-Translocating ATPases