Abstract
Immunity-related p47 guanosine triphosphatases (IRG) play a role in defense against intracellular pathogens. We found that the murine Irgm1 (LRG-47) guanosine triphosphatase induced autophagy and generated large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. We also identified a function for a human IRG protein in the control of intracellular pathogens and report that the human Irgm1 ortholog, IRGM, plays a role in autophagy and in the reduction of intracellular bacillary load.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Autophagy*
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Cell Line
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Cytosol / metabolism
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GTP-Binding Proteins / genetics
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GTP-Binding Proteins / physiology*
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HeLa Cells
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Humans
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Interferon-gamma / immunology
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Lysosomes / metabolism
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Lysosomes / microbiology
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Lysosomes / ultrastructure
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Macrophages / immunology*
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Macrophages / microbiology*
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Mice
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Microbial Viability
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Microtubule-Associated Proteins / metabolism
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Mycobacterium bovis / immunology*
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Mycobacterium bovis / physiology
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Phagosomes / metabolism
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Phagosomes / microbiology
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Phagosomes / ultrastructure*
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RNA, Small Interfering
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Transfection
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Vacuoles / metabolism
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Vacuoles / ultrastructure
Substances
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Ifi1 protein, mouse
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MAP1LC3A protein, human
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Microtubule-Associated Proteins
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RNA, Small Interfering
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Interferon-gamma
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GTP-Binding Proteins
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IRGM protein, human