Abstract
The gram-negative bacterium Bartonella henselae is capable of causing angiogenic lesions as a result of infection. Previously, it has been shown that B. henselae infection can result in production of the chemokine interleukin-8 (IL-8). In this study, we demonstrated that monocytes, endothelial cells, and hepatocytes produce IL-8 in response to B. henselae infection. We also investigated the role of IL-8 in B. henselae-induced endothelial cell proliferation and capillary tube formation. Both in vitro angiogenesis assays were IL-8 dependent. B. henselae-mediated inhibition of apoptosis, as indicated by gene expression of Bax and Bcl-2, was also shown to be IL-8 dependent in endothelial cells. Furthermore, infection of endothelial cells with B. henselae stimulated upregulation of the IL-8 chemokine receptor CXCR2. Infection of human endothelial cells by B. henselae resulting in IL-8 production likely plays a central role in the ability of this organism to cause angiogenesis during infection.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Angiomatosis, Bacillary / genetics
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Angiomatosis, Bacillary / immunology*
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Angiomatosis, Bacillary / pathology
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Apoptosis / genetics
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Autocrine Communication
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Bartonella henselae*
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Capillaries / growth & development
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Cell Proliferation
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Cells, Cultured
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Endothelium, Vascular / immunology
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Endothelium, Vascular / microbiology
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Endothelium, Vascular / pathology
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Gene Expression
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Hepatocytes / immunology
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Humans
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Immunoglobulin G / pharmacology
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Interleukin-8 / antagonists & inhibitors
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Interleukin-8 / genetics
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Interleukin-8 / physiology*
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Monocytes / immunology
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Neovascularization, Pathologic / genetics
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Neovascularization, Pathologic / immunology*
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Neovascularization, Pathologic / pathology
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Proto-Oncogene Proteins c-bcl-2 / genetics
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Receptors, Interleukin-8B / genetics
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Receptors, Interleukin-8B / metabolism*
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Up-Regulation
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bcl-2-Associated X Protein / genetics
Substances
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Immunoglobulin G
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Interleukin-8
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Proto-Oncogene Proteins c-bcl-2
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Receptors, Interleukin-8B
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bcl-2-Associated X Protein