Abstract
We compared the effect of control genes (CG): total Abelson (total-ABL), beta-2-microglobulin (B2M) and beta-glucuronidase (GUS), recommended in the Europe Against Cancer (EAC) program, on real-time BCR-ABL monitoring in patients with chronic myeloid leukemia (CML). We focused on the stability of CG expressions during therapy and the effect of the CGs on BCR-ABL ability to characterize the disease status and disease prognosis, issues that have not been addressed yet. The results showed B2M as a very convenient CG for BCR-ABL monitoring. On the contrary, the widely used total-ABL was not confirmed as appropriate for normalization of gene expression in CML.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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DNA, Complementary / genetics
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Female
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Fusion Proteins, bcr-abl / genetics*
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Gene Expression Regulation, Leukemic
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Glucuronidase / genetics*
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Humans
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / therapy
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Male
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Middle Aged
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Proto-Oncogene Proteins c-abl / genetics*
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RNA, Messenger / genetics
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RNA, Neoplasm / analysis
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Reverse Transcriptase Polymerase Chain Reaction
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beta 2-Microglobulin / genetics*
Substances
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DNA, Complementary
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RNA, Messenger
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RNA, Neoplasm
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beta 2-Microglobulin
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Fusion Proteins, bcr-abl
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Proto-Oncogene Proteins c-abl
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Glucuronidase