Abstract
Constitutive secretion of interleukin-6 (IL-6) has been proposed as a mechanism of transformation in multiple myeloma. We therefore studied 15 patients with this disease for rearrangements of the IL-6 and IL-6 receptor genes. Two patients with altered IL-6 genes were identified. Chromosome analysis revealed aberrations of chromosome 7, where the IL-6 gene resides, in only these two patients, but not in others without IL-6 rearrangements. In one of these patients, constitutive IL-6 m-RNA expression was observed. No alterations of IL-6 receptor genes were detected.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Blotting, Northern
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Blotting, Southern
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Bone Marrow / metabolism
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Bone Marrow / physiology
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Chromosomes, Human, Pair 7 / physiology
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Deoxyribonuclease EcoRI / metabolism
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Gene Rearrangement / genetics*
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Humans
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Interleukin-6 / genetics*
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Leukemia, Plasma Cell / blood
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Leukemia, Plasma Cell / genetics
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Leukocytes, Mononuclear / physiology
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Metaphase / physiology
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Molecular Sequence Data
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Multiple Myeloma / genetics*
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Polymerase Chain Reaction
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RNA / blood
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RNA, Messenger / biosynthesis
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Receptors, Immunologic / genetics*
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Receptors, Interleukin-6
Substances
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Interleukin-6
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RNA, Messenger
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Receptors, Immunologic
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Receptors, Interleukin-6
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RNA
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Deoxyribonuclease EcoRI