Abstract
Dbs is a Rho-specific guanine nucleotide exchange factor (RhoGEF) with in vitro exchange activity specific for RhoA and Cdc42. Like many RhoGEF family members, the in vivo exchange activity of Dbs is restricted in a cell-specific manner. Here we report the characterization of a novel scaffold protein (designated cell cycle progression protein 1 [Ccpg1]) that interacts with Dbs and modulates its in vivo exchange specificity. When coexpressed in mammalian cells, Ccpg1 binds to the Dbl homology/pleckstrin homology domain tandem motif of Dbs and inhibits its exchange activity toward RhoA, but not Cdc42. Expression of Ccpg1 correlates with the ability of Dbs to activate endogenous RhoA in cultured cells, and suppression of endogenous Ccpg1 expression potentiates Dbs exchange activity toward RhoA. The isolated Dbs binding domain of Ccpg1 is not sufficient to suppress Dbs exchange activity on RhoA, thus suggesting a regulatory interaction. Ccpg1 mediates recruitment of endogenous Src kinase into Dbs-containing complexes and interacts with the Rho family member Cdc42. Collectively, our studies suggest that Ccpg1 represents a new class of regulatory scaffold protein that can function as both an assembly platform for Rho protein signaling complexes and a regulatory protein which can restrict the substrate utilization of a promiscuous RhoGEF family member.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Binding Sites
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COS Cells
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Cell Cycle Proteins / metabolism
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Cell Line
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Cell Transformation, Neoplastic / metabolism
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Chlorocebus aethiops
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Enzyme Activation
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Escherichia coli / genetics
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Fibroblasts / cytology
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Fibroblasts / metabolism
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Genes, Reporter
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Glutathione Transferase / metabolism
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Guanine Nucleotide Exchange Factors / chemistry
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Guanine Nucleotide Exchange Factors / genetics
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Guanine Nucleotide Exchange Factors / metabolism*
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Hemagglutinins / chemistry
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Humans
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Luciferases / metabolism
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Mice
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Models, Biological
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Molecular Sequence Data
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Mutation
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NIH 3T3 Cells
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Protein Binding
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Protein Structure, Tertiary
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Proteins / chemistry
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Proteins / genetics
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Proteins / metabolism*
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Recombinant Fusion Proteins / metabolism
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Rho Guanine Nucleotide Exchange Factors
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Sequence Homology, Amino Acid
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cdc42 GTP-Binding Protein / chemistry
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cdc42 GTP-Binding Protein / genetics
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cdc42 GTP-Binding Protein / metabolism
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rhoA GTP-Binding Protein / chemistry
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rhoA GTP-Binding Protein / genetics
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rhoA GTP-Binding Protein / metabolism
Substances
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Ccpg1 protein, mouse
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Cell Cycle Proteins
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Guanine Nucleotide Exchange Factors
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Hemagglutinins
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Mcf2l protein, mouse
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Proteins
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Recombinant Fusion Proteins
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Rho Guanine Nucleotide Exchange Factors
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Luciferases
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Glutathione Transferase
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cdc42 GTP-Binding Protein
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rhoA GTP-Binding Protein