CTP:phosphoethanolamine cytidylyltransferase (ECT) is the enzyme that catalyzes the conversion of phosphoethanolamine to CDP-ethanolamine in the phosphatidylethanolamine-biosynthetic pathway (Kennedy pathway). ECT from Saccharomyces cerevisiae was crystallized by the sitting-drop vapour-diffusion method using PEG 4000 as precipitant. The crystals diffracted X-rays from a synchrotron-radiation source to 1.88 A resolution. The space group was assigned as primitive tetragonal, P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 66.3, c = 150.8 A. The crystals contain one ECT molecule in the asymmetric unit (V(M) = 2.2 A(3) Da(-1)), with a solvent content of 43%.