Abstract
The tumor suppressor gene PTEN, which encodes a multifunctional phosphatase protein, is mutated in a variety of human cancers. Several reports have indicated that it has growth-suppressive and proapoptosis properties and displayed an altered expression pattern during human oncogenesis. Overexpression of PTEN leads to decreasing cell growth and tumorigenicity in vitro and in vivo. In the present study, we further demonstrated that overexpression of PTEN mediated by adenovirus suppressed bladder cancer cell growth and significantly induced apoptosis, through downregulating of survivin and activating of caspase cascades. Our results indicate that Ad-PTEN exerts its tumor suppressive effect on bladder cancer cells through inhibiting survivin and upregulating caspase-related proteins. Thus Ad-PTEN may be potentially therapeutic for the treatment of bladder cancers.
MeSH terms
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Adenoviridae / genetics
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Apoptosis / physiology*
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Caspases / genetics
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Caspases / metabolism
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Cell Line, Tumor
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Cell Proliferation*
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Gene Expression Regulation, Enzymologic / physiology
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Gene Expression Regulation, Neoplastic / physiology
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Gene Transfer Techniques
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Genes, Tumor Suppressor
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Genetic Therapy / methods
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Humans
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Inhibitor of Apoptosis Proteins
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Microtubule-Associated Proteins / genetics
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Microtubule-Associated Proteins / metabolism*
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism*
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PTEN Phosphohydrolase / genetics
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PTEN Phosphohydrolase / metabolism*
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Survivin
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Urinary Bladder Neoplasms / genetics
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Urinary Bladder Neoplasms / metabolism*
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Urinary Bladder Neoplasms / pathology*
Substances
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BIRC5 protein, human
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Inhibitor of Apoptosis Proteins
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Microtubule-Associated Proteins
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Neoplasm Proteins
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Survivin
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PTEN Phosphohydrolase
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PTEN protein, human
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Caspases