The expression of FHIT, PCNA and EGFR in benign and malignant breast lesions

Br J Cancer. 2007 Jan 15;96(1):110-7. doi: 10.1038/sj.bjc.6603512. Epub 2006 Dec 12.

Abstract

Immunohistochemical staining for FHIT and PCNA proteins was carried out in 451 breast lesions showing nonproliferative benign breast disease (BBD) (n=263), proliferative BBD without atypia (n=128), proliferative BBD with atypia (n=11), carcinoma in situ (n=15) or invasive carcinoma (n=34) and for EGFR protein in a subset of 71 of these cases. FHIT underexpression was not detected in nonproliferative lesions, but occurred in 2% of proliferative BBD without atypia, 10% proliferative BBD with atypia, 27% of carcinoma in situ and 41% of invasive carcinoma, which suggests that it could be useful in assessing those carcinoma in situ lesions (ductal, DCIS and lobular, LCIS) that are more likely to progress to malignancy. Preliminary microarray comparisons on DCIS and invasive carcinoma samples dissected from formalin-fixed paraffin sections showed a consistent downregulation of two previously identified FHIT-related genes, caspase 1 and BRCA1 in lesions underexpressing FHIT.

MeSH terms

  • Acid Anhydride Hydrolases / biosynthesis*
  • Acid Anhydride Hydrolases / chemistry
  • Acid Anhydride Hydrolases / genetics
  • Breast Diseases / metabolism*
  • Breast Diseases / pathology
  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / pathology
  • Carcinoma, Ductal, Breast / metabolism*
  • Carcinoma, Ductal, Breast / pathology
  • Carcinoma, Intraductal, Noninfiltrating / metabolism*
  • Carcinoma, Intraductal, Noninfiltrating / pathology
  • ErbB Receptors / biosynthesis*
  • ErbB Receptors / chemistry
  • ErbB Receptors / genetics
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • Neoplasm Proteins / biosynthesis*
  • Neoplasm Proteins / chemistry
  • Neoplasm Proteins / genetics
  • Oligonucleotide Array Sequence Analysis / methods
  • Polymerase Chain Reaction / methods
  • Proliferating Cell Nuclear Antigen / biosynthesis*
  • Proliferating Cell Nuclear Antigen / chemistry
  • Proliferating Cell Nuclear Antigen / genetics
  • RNA / genetics
  • Sensitivity and Specificity
  • Staining and Labeling

Substances

  • Neoplasm Proteins
  • Proliferating Cell Nuclear Antigen
  • fragile histidine triad protein
  • RNA
  • ErbB Receptors
  • Acid Anhydride Hydrolases