Abstract
The normal epithelial cell-specific-1 (NES1)/Kallikrein 10 gene is proposed to be a novel putative tumor suppressor gene in several malignant diseases. The role of NES1 gene in gastric cancer has not been fully understood. Our study revealed that CpG island hypermethylation plays an important role in the downregulation of NES1 mRNA expression in gastric cancer. In situ hybridization showed that loss or reduction of NES1 mRNA expression is associated with differentiation level during tumor progression suggesting that NES1 inactivation might contribute to the malignant progression of human gastric cancers.
MeSH terms
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Antimetabolites, Antineoplastic / pharmacology
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Azacitidine / analogs & derivatives
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Azacitidine / pharmacology
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Cell Line, Transformed
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Cell Line, Tumor
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CpG Islands / genetics*
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DNA Methylation*
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Decitabine
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Down-Regulation / genetics
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Gene Expression Regulation, Neoplastic / drug effects
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Genetic Predisposition to Disease
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Humans
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In Situ Hybridization
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Kallikreins / genetics*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Stomach Neoplasms / genetics
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Stomach Neoplasms / pathology*
Substances
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Antimetabolites, Antineoplastic
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RNA, Messenger
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Decitabine
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KLK10 protein, human
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Kallikreins
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Azacitidine