Objectives: To investigate the association between IL1B polymorphisms and risk of gastric cancer in a Chinese population, seven SNPs in the IL1B gene were selected for this study.
Methods: A multiplex genotyping method, which is based on adapter ligation and allele-specific amplification, was established to type seven SNPs on the IL1B gene simultaneously. One hundred and forty-one non-cancer outpatients and 97 patients with gastric cancer were genotyped, and the relation between IL1B polymorphisms and gastric cancer was statistically analyzed.
Results: The seven SNPs were successfully typed and the results were consistent with those obtained by both Sanger's sequencing and PCR-RFLP. Handling with statistical analysis, we observed significantly different genotype frequencies of 0794C>T (chi(2)=6.11, P=0.05), 1274C>T (chi(2)=6.86, P=0.03) and 2143T>C (chi(2)=6.86, P=0.03) between patients and controls.
Conclusion: ALM-ASA is a potential method for multiplex SNP typing with a low consumption of genomic DNA. SNPs 0794C>T, 1274C>T, and 2143T>C are associated with gastric cancer.