Abstract
A murine monoclonal antibody (M31-15) was identified using the penetration-inhibiting assay of a human lung adenocarcinoma cell line (MAC10) and remarkably inhibited the phagokinetic tract motility of various cancer cell lines. The antigen, motility-related protein (MRP-1), recognized by M31-15, was 25- and 28-kD proteins, and M31-15 was used to isolate a cDNA clone from a human breast carcinoma cDNA library. Sequence analysis revealed that MRP-1 had strong similarity with a B cell surface antigen (CD37), a melanoma-associated antigen (ME491), the target of an antiproliferative antibody (TAPA-1), a human tumor-associated antigen (CO-029), and the Sm23 antigen of the trematode parasite Schistosoma mansoni.
MeSH terms
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Adenocarcinoma / immunology
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / immunology*
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Antigens, CD*
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Antigens, Surface / analysis*
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Antigens, Surface / genetics
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Antigens, Surface / immunology
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Base Sequence
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Cell Movement*
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DNA / isolation & purification
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Epitopes / analysis
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Humans
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Immunohistochemistry
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Membrane Glycoproteins*
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Proteins / analysis*
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Tetraspanin 29
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Tumor Cells, Cultured
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, Surface
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CD9 protein, human
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Cd9 protein, mouse
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Epitopes
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Membrane Glycoproteins
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Proteins
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Tetraspanin 29
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DNA
Associated data
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GENBANK/S64904
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GENBANK/S69611
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GENBANK/X60111
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GENBANK/X61557
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GENBANK/X61558
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GENBANK/X61559
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GENBANK/X61560
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GENBANK/X61561
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GENBANK/X61562
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GENBANK/X61563