Abstract
Recent studies suggest that NGAL (neutrophil gelatinase-associated lipocalin) is a novel iron transporter with functions distinct from that of transferrin and mediates a new iron-delivery pathway. To get a better understanding of NGAL function in oesophageal carcinoma, we analysed the expression of NGAL receptors in oesophageal carcinoma cells and identified a novel spliced variant designated NgalR-3. When expressed in a heterologous system, the protein produced from this novel spliced variant exhibits the biochemical characteristics of interaction and co-localization with NGAL protein in vivo. This new finding suggests that NgalR-3 may act as a potential NGAL receptor and play a role in NGAL-mediated iron transport in oesophageal carcinoma.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acute-Phase Proteins / genetics*
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Acute-Phase Proteins / metabolism*
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Alternative Splicing / genetics*
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Amino Acid Sequence
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Animals
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Cell Line
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Cell Membrane / metabolism
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Chlorocebus aethiops
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Cloning, Molecular
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Conserved Sequence
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DNA, Complementary / genetics
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Esophageal Neoplasms / enzymology*
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Esophageal Neoplasms / genetics*
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Neoplastic
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Genetic Variation / genetics*
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Humans
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Lipocalin-2
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Lipocalins
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Molecular Sequence Data
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Organic Cation Transport Proteins
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Protein Binding
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins / metabolism*
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RNA, Messenger / genetics
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Receptors, Peptide / genetics*
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Receptors, Peptide / metabolism*
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Sequence Alignment
Substances
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Acute-Phase Proteins
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DNA, Complementary
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LCN2 protein, human
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Lipocalin-2
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Lipocalins
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Organic Cation Transport Proteins
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Proto-Oncogene Proteins
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RNA, Messenger
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Receptors, Peptide
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SLC22A17 protein, human