Identification of trophinin as an enhancer for cell invasion and a prognostic factor for early stage lung cancer

Kuan-Yu Chen; Yuan-Chii Gladys Lee; Jin-Mei Lai; Yih-Leong Chang; Yung-Chie Lee; Chong-Jen Yu; Chi-Ying F Huang; Pan-Chyr Yang

doi:10.1016/j.ejca.2006.09.029

Identification of trophinin as an enhancer for cell invasion and a prognostic factor for early stage lung cancer

Eur J Cancer. 2007 Mar;43(4):782-90. doi: 10.1016/j.ejca.2006.09.029. Epub 2007 Jan 24.

Authors

Kuan-Yu Chen¹, Yuan-Chii Gladys Lee, Jin-Mei Lai, Yih-Leong Chang, Yung-Chie Lee, Chong-Jen Yu, Chi-Ying F Huang, Pan-Chyr Yang

Affiliation

¹ Department of Internal Medicine, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, Taiwan.

PMID: 17254769
DOI: 10.1016/j.ejca.2006.09.029

Abstract

The explosion of microarray studies has promised to shed light on the identification of disease markers. To find novel prognostic factors, we used the expression profile of a poor prognostic factor of lung cancer, survivin (BIRC5), as a template to search for and compare transcriptome expression profiles in a lung adenocarcinoma microarray dataset. Trophinin (TRO) was identified as one of the best-correlated genes. The trophinin expression in lung cancer specimens was examined by immunohistochemical staining. The role of trophinin in cancer metastasis was further investigated by approaches of overexpression and knock down with small interfering RNA (siRNA). For stage I lung adenocarcinoma, the patients without trophinin expression had a better overall and disease-free survival. Overexpression of trophinin increases cell invasion ability and knock down with siRNA inhibits cell invasion. Through a combination of data mining and biochemical assays, we identified trophinin, which could enhance cell invasion, as a novel prognostic factor for early stage lung cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma / diagnosis*
Adenocarcinoma / genetics
Aged
Biomarkers, Tumor / metabolism*
Cell Adhesion Molecules / genetics
Cell Adhesion Molecules / metabolism*
Cell Line, Tumor
Female
Humans
Immunohistochemistry
Inhibitor of Apoptosis Proteins
Lung Neoplasms / diagnosis*
Lung Neoplasms / genetics
Male
Microarray Analysis / methods
Microtubule-Associated Proteins / genetics
Microtubule-Associated Proteins / metabolism*
Middle Aged
Neoplasm Invasiveness / diagnosis*
Neoplasm Invasiveness / genetics
Neoplasm Metastasis / diagnosis
Neoplasm Metastasis / genetics
Neoplasm Proteins / genetics
Neoplasm Proteins / metabolism*
Neoplasm Recurrence, Local / diagnosis
Neoplasm Recurrence, Local / genetics
Prognosis
RNA, Small Interfering / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Survival Analysis
Survivin

Substances

BIRC5 protein, human
Biomarkers, Tumor
Cell Adhesion Molecules
Inhibitor of Apoptosis Proteins
Microtubule-Associated Proteins
Neoplasm Proteins
RNA, Small Interfering
Survivin
TRO protein, human