Abstract
We show that a glycerophosphodiester phosphodiesterase homolog, GDE2, is widely expressed in brain tissues including primary neurons, and that the expression of GDE2 in neuroblastoma Neuro2A cells is significantly upregulated during neuronal differentiation by retinoic acid (RA) treatment. Stable expression of GDE2 resulted in neurite formation in the absence of RA, and GDE2 accumulated at the regions of perinuclear and growth cones in Neuro2A cells. Furthermore, a loss-of-function of GDE2 in Neuro2A cells by RNAi blocked RA-induced neurite formation. These results demonstrate that GDE2 expression during neuronal differentiation plays an important role for growing neurites.
MeSH terms
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Animals
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Brain / drug effects
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Brain / enzymology
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Brain / metabolism
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Enzyme Activation / drug effects
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Extracellular Signal-Regulated MAP Kinases / metabolism
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Gene Expression / drug effects
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Gene Expression Profiling
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Gene Expression Regulation, Enzymologic / drug effects
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Gene Expression Regulation, Neoplastic / drug effects
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Humans
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Mice
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Neurites / drug effects*
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Neurites / enzymology*
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Neuroblastoma / enzymology*
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Neuroblastoma / pathology*
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Phosphoric Diester Hydrolases / genetics
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Phosphoric Diester Hydrolases / metabolism*
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Protein Transport / drug effects
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA, Small Interfering / metabolism
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Tretinoin / pharmacology*
Substances
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Membrane Proteins
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RNA, Messenger
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RNA, Small Interfering
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Tretinoin
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Extracellular Signal-Regulated MAP Kinases
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Phosphoric Diester Hydrolases
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glycerophosphodiester phosphodiesterase