Abstract
A rapid multiplex PCR approach was developed to detect the bft gene subtypes in Bacteroides fragilis clinical isolates. This technique could be used to look at the epidemiology of enterotoxigenic strains of B. fragilis in clinical infections and whether there is a correlation between disease and the presence of B. fragilis enterotoxin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Toxins / genetics*
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Bacteroides Infections / microbiology
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Bacteroides fragilis / classification*
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Bacteroides fragilis / genetics*
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Bacteroides fragilis / isolation & purification
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Electrophoresis, Agar Gel
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Female
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Genes, Bacterial*
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Humans
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Metalloendopeptidases / genetics*
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Molecular Epidemiology / methods
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Polymerase Chain Reaction / methods*
Substances
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Bacterial Toxins
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Bacteroides fragilis toxin
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Metalloendopeptidases