Abstract
Calcitonin gene-related peptide is a 37 amino acid neuropeptide. Although calcitonin gene-related peptide activates a G-protein-coupled receptor, recent evidence suggests that calcitonin gene-related peptide induces more complex signaling cascades including the activation of MAP kinases [Eur J Pharmacol; 389:125-130 (2000), Neuropeptides; 34:229-233 (2000)]. However, the molecular mechanisms of this activation still remain to be elucidated. For the first time we applied a proteomics approach in order to identify molecular targets of calcitonin gene-related peptide downstream signaling in the neuroblastoma cell line SK-N-MC and identified proteins that changed either their expression, location, or their post-translational modifications in a time-dependent manner after calcitonin gene-related peptide stimulation.
MeSH terms
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Amino Acid Sequence
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Calcitonin Gene-Related Peptide / pharmacology*
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Cell Line, Tumor / cytology
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Cell Line, Tumor / drug effects
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Cell Proliferation / drug effects*
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Gene Expression Profiling*
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Humans
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Mitogen-Activated Protein Kinases / genetics
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Mitogen-Activated Protein Kinases / metabolism
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Molecular Sequence Data
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Neuroblastoma / metabolism
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Neuroblastoma / pathology
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Neuropeptides / genetics
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Neuropeptides / metabolism
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Protein Processing, Post-Translational / drug effects*
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Protein Processing, Post-Translational / physiology
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Proteomics*
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Receptors, Calcitonin Gene-Related Peptide / genetics
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Receptors, Calcitonin Gene-Related Peptide / metabolism
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Signal Transduction / drug effects*
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Signal Transduction / physiology
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Time Factors
Substances
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Neuropeptides
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Receptors, Calcitonin Gene-Related Peptide
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Mitogen-Activated Protein Kinases
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Calcitonin Gene-Related Peptide