Abstract
Recent studies have demonstrated that histone methylation can be dynamically regulated through active demethylation. However, no demethylase specific to histone H3 trimethyl-Lys4 (H3K4me3) has been identified. Here we report that the Drosophila melanogaster protein 'little imaginal discs' (Lid), a JmjC domain-containing trithorax group protein, can demethylate H3K4me3. Consistent with its genetic classification, Lid positively regulates Hox gene expression in S2 cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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DNA-Binding Proteins / metabolism*
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Drosophila Proteins / chemistry
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Drosophila Proteins / metabolism*
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Drosophila melanogaster / cytology
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Drosophila melanogaster / enzymology*
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Histone Demethylases
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Histone-Lysine N-Methyltransferase / chemistry
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Histone-Lysine N-Methyltransferase / metabolism*
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Histones / metabolism*
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Larva / cytology
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Lysine / metabolism*
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Methylation
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Mutant Proteins / metabolism
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Protein Structure, Tertiary
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Protein Transport
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RNA Polymerase II / metabolism
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Substrate Specificity
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Transcription Factors / metabolism*
Substances
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DNA-Binding Proteins
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Drosophila Proteins
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Histones
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Mutant Proteins
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Transcription Factors
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Trl protein, Drosophila
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Histone Demethylases
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Lid protein, Drosophila
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Histone-Lysine N-Methyltransferase
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RNA Polymerase II
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Lysine