Strain collections and genetic nomenclature

Methods Enzymol. 2007:421:3-8. doi: 10.1016/S0076-6879(06)21001-2.

Abstract

The ease of rapidly accumulating a large number of mutants requires careful bookkeeping to avoid confusing one mutant with another. Each mutant constructed should be assigned a strain number. Strain numbers usually consist of two to three capital letters designating the lab where they were constructed and a serial numbering of the strains in a central laboratory collection. Every mutation should be assigned a name that corresponds to a particular gene or phenotype, and an allele number that identifies each specific isolate. When available for a particular group of bacteria, genetic stock centers are the ultimate resources for gene names and allele numbers. Examples include the Salmonella Genetic Stock Centre ( http://www.ucalgary.ca/~kesander/), and the E. coli Genetic Stock Center (http://cgsc.biology.yale.edu/). It is also important to indicate how the strain was constructed, the parental (recipient) strain, and the source of any donor DNA transferred into the recipient strain (Maloy et al., 1996).

Publication types

  • Review

MeSH terms

  • Alleles
  • Chromosomes, Bacterial
  • DNA Transposable Elements
  • DNA, Bacterial
  • Genes, Bacterial*
  • Mutation
  • Plasmids
  • Terminology as Topic*

Substances

  • DNA Transposable Elements
  • DNA, Bacterial