Abstract
This study determined the conservation and protective efficacy of a 48 kDa nontypable Haemophilus influenzae (NTHi) protein (P48). This protein was highly conserved across the strains of NTHi examined and mucosal immunization with recombinant P48 (rP48) significantly reduced the numbers of viable NTHi recovered from the lung following challenge. rP48 induced predominantly an IgG2a antibody response that correlated with the reduction in the number of viable NTHi in the lung. These antibodies were not bactericidal against NTHi. The results suggest that P48 warrants further investigation as a vaccine component for NTHi disease.
MeSH terms
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Adult
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Aged
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Amino Acid Sequence
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Animals
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Antibodies, Bacterial / blood
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Antibodies, Bacterial / immunology
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Antibody Specificity
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Bacterial Proteins / genetics
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Bacterial Proteins / immunology*
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Bacterial Proteins / isolation & purification
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Child
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Child, Preschool
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Cloning, Molecular
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Female
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Haemophilus Infections / immunology
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Haemophilus Infections / microbiology
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Haemophilus Infections / prevention & control
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Haemophilus Vaccines / genetics
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Haemophilus Vaccines / immunology
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Haemophilus Vaccines / pharmacology*
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Haemophilus influenzae / genetics
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Haemophilus influenzae / immunology*
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Haemophilus influenzae / isolation & purification
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Humans
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Infant
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Male
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Middle Aged
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Molecular Sequence Data
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Rats
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Rats, Wistar
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Sequence Alignment
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / microbiology
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Vaccines, Synthetic / pharmacology
Substances
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Antibodies, Bacterial
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Bacterial Proteins
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Haemophilus Vaccines
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Vaccines, Synthetic