Subtyping method for Escherichia coli shiga toxin (verocytotoxin) 2 variants and correlations to clinical manifestations

Søren Persson; Katharina E P Olsen; Steen Ethelberg; Flemming Scheutz

doi:10.1128/JCM.02591-06

Subtyping method for Escherichia coli shiga toxin (verocytotoxin) 2 variants and correlations to clinical manifestations

J Clin Microbiol. 2007 Jun;45(6):2020-4. doi: 10.1128/JCM.02591-06. Epub 2007 Apr 19.

Authors

Søren Persson¹, Katharina E P Olsen, Steen Ethelberg, Flemming Scheutz

Affiliation

¹ Department of Bacteriology, Mycology and Parasitology, The National Reference Laboratory for Enteropathogenic Bacteria, Unit of Gastrointestinal Infections, Statens Serum Institut, Artillerivej 5, Building 37B, 2300 Copenhagen S, Denmark. SPN@ssi.dk

Abstract

Shiga toxin 2 (Stx2) from Shiga toxin-producing Escherichia coli (STEC) was subtyped by a method involving partial sequencing of the stxAB2 operon. Of 255 strains from the Danish STEC cohort, all 20 cases of hemolytic-uremic syndrome were associated with subtype Stx2 (11 cases), subtype Stx2c (1 case), or the two combined (8 cases).

MeSH terms

Amino Acid Sequence
Child
Denmark
Diarrhea / microbiology
Diarrhea / physiopathology*
Escherichia coli / classification*
Escherichia coli / genetics
Escherichia coli / pathogenicity*
Escherichia coli Infections / microbiology
Escherichia coli Infections / physiopathology
Genetic Variation*
Hemolytic-Uremic Syndrome / microbiology
Hemolytic-Uremic Syndrome / physiopathology*
Humans
Molecular Sequence Data
Operon
Phylogeny
Sequence Analysis, DNA
Shiga Toxin 2 / chemistry
Shiga Toxin 2 / classification*
Shiga Toxin 2 / genetics
Shiga Toxin 2 / metabolism

Substances

Shiga Toxin 2

Associated data

GENBANK/DQ035774
GENBANK/DQ059012
GENBANK/DQ235775