ALK-mediated Na+/H+ exchanger-dependent intracellular alkalinization: does it matter for oncogenesis?

Haematologica. 2007 May;92(5):706-7. doi: 10.3324/haematol.10923.

Abstract

In this study we investigated the relevance of the oncogenic protein NPM-ALK in regulating cellular pH (pHi) through the modulation of Na+/H+ exchanger 1 (NHE1)-activity and the consequences of pHi pharmacological manipulation in cells expressing NPM-ALK.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine / analysis
  • Ammonia / analysis
  • Animals
  • Cell Division
  • Cell Line, Tumor / metabolism
  • Cell Transformation, Neoplastic / metabolism*
  • Chromans / pharmacology*
  • Culture Media, Conditioned / chemistry
  • Drug Screening Assays, Antitumor
  • Glutamic Acid / metabolism
  • Humans
  • Hydrogen-Ion Concentration*
  • Intracellular Fluid / chemistry*
  • Lymphoma, Large B-Cell, Diffuse / pathology*
  • Mice
  • NIH 3T3 Cells / drug effects
  • NIH 3T3 Cells / ultrastructure
  • Protein-Tyrosine Kinases / genetics
  • Protein-Tyrosine Kinases / physiology*
  • Sodium-Hydrogen Exchangers / antagonists & inhibitors
  • Sodium-Hydrogen Exchangers / physiology*
  • Thiazolidinediones / pharmacology*
  • Transfection
  • Troglitazone

Substances

  • Chromans
  • Culture Media, Conditioned
  • Sodium-Hydrogen Exchangers
  • Thiazolidinediones
  • Glutamic Acid
  • Ammonia
  • p80(NPM-ALK) protein
  • Protein-Tyrosine Kinases
  • Troglitazone
  • Alanine