Abstract
Background:
IL-9 might play a critical role in pathogenesis and development of atopic asthma, but there are few reports on allergen-specific IL-9 production by peripheral blood mononuclear cells (PBMCs) obtained from adult asthmatics.
Methods:
PBMCs were obtained from adult atopic asthmatics and incubated with Dermatophagoides farinae(Der f) extract for the designated time periods. The resulting supernatants were assayed for IL-9 by specific sandwich ELISA.
Results:
IL-9 production was detectable on day 2 and reached maximum on day 6 after stimulation of PBMCs with Der f extract. IL-9 production in response to Der f extract increased in a dose-dependent manner. CD2- or CD4-bearing cell depletion completely abolished IL-9 production by PBMCs, while CD8-bearing cell depletion did not affect it.
Conclusion:
CD4+ lymphocytes are the principal source of IL-9 produced by PBMCs of adult atopic asthmatics.
MeSH terms
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Adult
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Animals
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Antigens, Dermatophagoides / immunology*
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Antigens, Dermatophagoides / pharmacology
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Asthma / blood*
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Asthma / immunology
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CD2 Antigens / immunology
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CD4 Antigens / immunology
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CD4-Positive T-Lymphocytes / drug effects
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CD4-Positive T-Lymphocytes / metabolism*
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Cells, Cultured / drug effects
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Cells, Cultured / metabolism
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Dermatophagoides farinae / immunology
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Dose-Response Relationship, Immunologic
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Enzyme-Linked Immunosorbent Assay
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Female
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Gene Expression Regulation / genetics
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Gene Expression Regulation / immunology
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Humans
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Interleukin-13 / biosynthesis
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Interleukin-13 / genetics
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Interleukin-5 / biosynthesis
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Interleukin-5 / genetics
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Interleukin-9 / biosynthesis*
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Interleukin-9 / genetics
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Leukocytes, Mononuclear / drug effects
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Leukocytes, Mononuclear / metabolism*
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Lymphocyte Depletion
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Male
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Tissue Extracts / immunology
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Tissue Extracts / pharmacology
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Up-Regulation / genetics
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Up-Regulation / immunology
Substances
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Antigens, Dermatophagoides
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CD2 Antigens
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CD4 Antigens
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IL5 protein, human
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IL9 protein, human
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Interleukin-13
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Interleukin-5
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Interleukin-9
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Tissue Extracts