False negative histochemical reaction for myophosphorylase activity in fulminant sepsis due to methicillin resistant Staphylococcus aureus

Neuromuscul Disord. 2007 Dec;17(11-12):983-5. doi: 10.1016/j.nmd.2007.07.008. Epub 2007 Aug 24.

Abstract

We report a false negative histochemical reaction for myophosphorylase in the case of an 11 year old with fulminant Staphylococcus aureus. Due to increased creatine kinase levels and marked myoglobinuria a muscle biopsy was performed prior to death. The biopsy revealed rhabdomyolysis, glycogen depletion and absent myophosphorylase reactivity. Subsequent myophosphorylase quantification was normal. This unique case of a false negative myophosphorylase histochemical reaction is apparently related to sepsis.

Publication types

  • Case Reports

MeSH terms

  • Autopsy
  • Biomarkers / analysis
  • Biomarkers / metabolism
  • Child
  • Creatine Kinase / analysis
  • Creatine Kinase / blood
  • Diagnosis, Differential
  • False Negative Reactions
  • Fatal Outcome
  • Glycogen / deficiency
  • Glycogen Phosphorylase, Muscle Form / analysis
  • Glycogen Phosphorylase, Muscle Form / metabolism*
  • Histocytochemistry
  • Humans
  • Male
  • Methicillin Resistance
  • Muscle, Skeletal / enzymology*
  • Muscle, Skeletal / pathology
  • Muscle, Skeletal / physiopathology
  • Myocardium / enzymology
  • Myocardium / pathology
  • Rhabdomyolysis / diagnosis*
  • Rhabdomyolysis / enzymology*
  • Rhabdomyolysis / microbiology
  • Sepsis / complications*
  • Sepsis / physiopathology
  • Staphylococcal Infections / complications*
  • Staphylococcal Infections / physiopathology
  • Staphylococcus aureus

Substances

  • Biomarkers
  • Glycogen
  • Glycogen Phosphorylase, Muscle Form
  • Creatine Kinase