Abstract
NF-kappaB is a family of transcription factors that have been shown to be elevated in a variety of tumor types and in some cases central to their survival and growth. Here we present evidence that U-87 MG and U-118 MG growth is regulated by NF-kappaB and controlled by PDGF. NF-kappaB activity was suppressed by a dominant negative mutant of the human PDGF type beta receptor and PDGF-B chain neutralizing antibodies. Creation of cell lines that had inducible expression of shRNAs directed against either c-Rel or RelA inhibited growth almost 90% indicating that NF-kappaB plays a central role in glioblastoma growth.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Astrocytoma / genetics
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Astrocytoma / pathology*
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Cell Line, Tumor
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Cell Proliferation* / drug effects
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Disease Progression
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Gene Expression Regulation, Neoplastic / drug effects
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Genes, rel
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Glioblastoma / genetics
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Glioblastoma / pathology*
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Humans
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NF-kappa B / antagonists & inhibitors
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NF-kappa B / physiology*
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Platelet-Derived Growth Factor / genetics
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RNA Interference
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RNA, Small Interfering / pharmacology
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Transcription Factor RelA / genetics
Substances
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NF-kappa B
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Platelet-Derived Growth Factor
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RELA protein, human
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RNA, Small Interfering
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Transcription Factor RelA