Abstract
Angiopoietin-like protein (Angptl) 1, a member of the angiopoietin-related protein family, modulates angiogenesis but little else is known of its physiological role. We found that angptl1 was upregulated at the 7th day after focal cerebral ischemia in normal mice. In order to understand the role of angptl1 in cerebral infarction, we induced focal cerebral ischemia in normal and glial fibrillary acidic protein promoter-angptl1 transgenic mice. In the transgenic mice without ischemia, overexpression of angptl1 in the whole brain led to a decrease in cortical microvascular density. Following focal cerebral ischemia, edema, but not infarct size, was less in transgenic mice relative to wild type littermates. This effect might be due to a reduction in the blood brain barrier breakdown, as confirmed by a decrease in Evans Blue leakage in the early post-ischemic phase. We conclude that angptl1 may have a beneficial role in the preservation of vascular integrity following focal cerebral ischemia.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Angiopoietin-Like Protein 1
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Angiopoietin-like Proteins
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Angiopoietins / genetics
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Angiopoietins / metabolism*
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Animals
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Blood-Brain Barrier / metabolism*
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Blood-Brain Barrier / physiopathology
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Brain Edema / genetics
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Brain Edema / metabolism*
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Brain Edema / physiopathology
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Brain Infarction / genetics
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Brain Infarction / metabolism*
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Brain Infarction / physiopathology
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Brain Ischemia / genetics
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Brain Ischemia / metabolism*
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Brain Ischemia / physiopathology
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Cerebral Arteries / metabolism
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Cerebral Arteries / physiopathology
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Evans Blue
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Glial Fibrillary Acidic Protein / genetics
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Humans
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Intercellular Signaling Peptides and Proteins / genetics
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Intercellular Signaling Peptides and Proteins / metabolism*
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Mice
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Mice, Transgenic
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Microcirculation / metabolism
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Microcirculation / physiopathology
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Promoter Regions, Genetic / genetics
Substances
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ANGPTL1 protein, human
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Angiopoietin-Like Protein 1
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Angiopoietin-like Proteins
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Angiopoietins
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Angptl1 protein, mouse
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Glial Fibrillary Acidic Protein
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Intercellular Signaling Peptides and Proteins
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Evans Blue