Mutations and haplotype diversity in 70 Portuguese G6PD-deficient individuals: an overview on the origin and evolution of mutated alleles

Haematologica. 2007 Dec;92(12):1713-4. doi: 10.3324/haematol.11670.

Abstract

G6PD deficiency mutational profile and haplotype diversity using 6 RFLPs (FokI/PvuII/BspHI/PstI/BclI/NlaIII) and a (CTT)(n) microsatellite, were investigated in 70 G6PD-deficient Portuguese individuals. All but one G6PD A-(376G/202A) variants (44/45) have a single haplotype (+/+/-/+/-/+/195). G6PD Betica(376G/968C) alleles (n=10) have a single RFLP haplotype (+/-/-/+/-/+) and 4 different (CTT)(n) repeats. Age estimates based on microsatellite variation suggest that Betica mutation arose 900 generations ago. G6PD SantaMaria(376G/542T) allele was found on haplotype (+/-/-/+/-/+/201) and 10 G6PD variants on RFLP haplotypes (-/-/+/+/-/-), (-/-/+/+/-/+) and (-/-/+/+/+/+).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Evolution, Molecular*
  • Female
  • Glucosephosphate Dehydrogenase / genetics*
  • Glucosephosphate Dehydrogenase Deficiency / genetics*
  • Haplotypes
  • Humans
  • Male
  • Microsatellite Repeats / genetics*
  • Point Mutation*
  • Polymorphism, Restriction Fragment Length*
  • Portugal

Substances

  • Glucosephosphate Dehydrogenase
  • glucose-6-phosphate dehydrogenase A-