Abstract
RNA polymerase II (Pol II) transcribes genes that encode proteins and noncoding small nuclear RNAs (snRNAs). The carboxyl-terminal repeat domain (CTD) of the largest subunit of mammalian RNA Pol II, comprising tandem repeats of the heptapeptide consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7, is required for expression of both gene types. We show that mutation of serine-7 to alanine causes a specific defect in snRNA gene expression. We also present evidence that phosphorylation of serine-7 facilitates interaction with the snRNA gene-specific Integrator complex. These findings assign a biological function to this amino acid and highlight a gene type-specific requirement for a residue within the CTD heptapeptide, supporting the existence of a CTD code.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Alanine
-
Amino Acid Sequence
-
Cell Line
-
Consensus Sequence
-
Gene Expression Regulation*
-
Heterogeneous-Nuclear Ribonucleoproteins / genetics
-
Humans
-
Mutation
-
Oligopeptides / chemistry
-
Oligopeptides / metabolism
-
Phosphorylation
-
Protein Structure, Tertiary
-
Protein Subunits / genetics
-
Protein Subunits / metabolism
-
RNA Polymerase II / chemistry
-
RNA Polymerase II / genetics
-
RNA Polymerase II / metabolism*
-
RNA Processing, Post-Transcriptional
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
RNA, Small Nuclear / genetics*
-
Serine / metabolism*
-
Templates, Genetic
-
Transcription, Genetic*
Substances
-
Heterogeneous-Nuclear Ribonucleoproteins
-
Oligopeptides
-
Protein Subunits
-
RNA, Messenger
-
RNA, Small Nuclear
-
U2 small nuclear RNA
-
Serine
-
RNA Polymerase II
-
Alanine