Abstract
We have established a novel human immunodeficiency virus (HIV) tandem-reporter assay using HIV receptor-transduced NP-2 cells with long terminal repeat-controlled beta-galactosidase, inserted internal ribosome entry site, and secretary alkaline phosphatase genes. This assay allows users to detect replication of clinical isolates, indicating its useful application as an HIV phenotypic assay.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / genetics
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Alkaline Phosphatase / metabolism
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Anti-HIV Agents / pharmacology
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Cell Line
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Genes, Reporter
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HIV / drug effects
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HIV / growth & development
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HIV / isolation & purification*
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HIV Infections / diagnosis*
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HIV Long Terminal Repeat / genetics
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Humans
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Microbial Sensitivity Tests / methods*
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Virology / methods*
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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Anti-HIV Agents
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Alkaline Phosphatase
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beta-Galactosidase