Dual localization of the RNA binding protein CUGBP-1 to stress granule and perinucleolar compartment

Ken Fujimura; Fumi Kano; Masayuki Murata

doi:10.1016/j.yexcr.2007.10.024

Dual localization of the RNA binding protein CUGBP-1 to stress granule and perinucleolar compartment

Exp Cell Res. 2008 Feb 1;314(3):543-53. doi: 10.1016/j.yexcr.2007.10.024. Epub 2007 Nov 12.

Authors

Ken Fujimura¹, Fumi Kano, Masayuki Murata

Affiliation

¹ Department of Life Sciences, Graduate School of Liberal Arts and Sciences, University of Tokyo, Tokyo, 3-8-1 Komaba, Meguro-ku, 153-8902, Japan. kfuji@bio.c.u-tokyo.ac.jp <kfuji@bio.c.u-tokyo.ac.jp>

PMID: 18164289
DOI: 10.1016/j.yexcr.2007.10.024

Abstract

The mRNA-binding protein CUGBP-1 is a multi-faceted factor, involved in a wide range of biological processes including splicing, translation initiation and mRNA degradation. Here we show that CUGBP-1 is a novel constituent of stress granule (SG), the translational silencing machinery assembled in response to environmental stress. CUGBP-1 was rapidly routed to SGs upon exposure to a variety of environmental stress, and actively shuttles between the nucleus and SGs. The linker domain located between the second and third RNA recognition motifs (RRMs) was found to be essential for the recruitment of CUGBP-1 to SGs. Importantly, we discovered that the linker domain is also required to direct CUGBP-1 to another subcellular structure, perinucleolar compartment (PNC). These results demonstrate the dynamic behavior of CUGBP-1 during stress response and that the linker region, in concert with RRMs, plays a significant role in defining its subcellular localization and dynamics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Active Transport, Cell Nucleus / genetics
Amino Acid Motifs / genetics
CELF1 Protein
Cell Compartmentation / genetics*
Cell Nucleolus / genetics
Cell Nucleolus / metabolism*
Cell Nucleolus / ultrastructure
Cell Nucleus / genetics
Cell Nucleus / metabolism
Cell Nucleus / ultrastructure
Cytoplasmic Granules / genetics
Cytoplasmic Granules / metabolism*
Cytoplasmic Granules / ultrastructure
HeLa Cells
Humans
Poly(A)-Binding Proteins / genetics
Poly(A)-Binding Proteins / metabolism
Protein Structure, Tertiary / genetics
Protein Transport / genetics
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism*
Stress, Physiological / genetics
Stress, Physiological / metabolism*
Stress, Physiological / physiopathology
T-Cell Intracellular Antigen-1

Substances

CELF1 Protein
CELF1 protein, human
Poly(A)-Binding Proteins
RNA, Messenger
RNA-Binding Proteins
T-Cell Intracellular Antigen-1
TIA1 protein, human