Abstract
A cDNA library prepared from a human glioblastoma cell line has been introduced into a budding yeast strain that lacks CLN1 and CLN2 and is conditionally deficient for CLN3 function. We rescued a gene that we call cyclin D1. It is related to A-, B-, and CLN-type cyclins, but appears to define a new subclass within the cyclin gene family. Transcription of the cyclin D1 gene gives rise to two major transcripts through alternative polyadenylation. The cyclin D1 gene transcript and its 34 kd product are both abundant in the glioblastoma cell line of origin.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Biological Evolution
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Blotting, Western
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Cell Line
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Cloning, Molecular / methods
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Cyclins / genetics*
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DNA, Neoplasm / genetics
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DNA, Neoplasm / isolation & purification
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Gene Library
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Genes
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Glioma
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HeLa Cells / physiology
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Humans
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Molecular Sequence Data
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RNA, Neoplasm / genetics
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RNA, Neoplasm / isolation & purification
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Saccharomyces cerevisiae / genetics
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Sequence Homology, Nucleic Acid
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Transcription, Genetic
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Tripeptidyl-Peptidase 1
Substances
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Cyclins
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DNA, Neoplasm
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RNA, Neoplasm
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Tripeptidyl-Peptidase 1