The yeast p24 complex is required for the formation of COPI retrograde transport vesicles from the Golgi apparatus

Auxiliadora Aguilera-Romero; Joanna Kaminska; Anne Spang; Howard Riezman; Manuel Muñiz

doi:10.1083/jcb.200710025

The yeast p24 complex is required for the formation of COPI retrograde transport vesicles from the Golgi apparatus

J Cell Biol. 2008 Feb 25;180(4):713-20. doi: 10.1083/jcb.200710025. Epub 2008 Feb 18.

Authors

Auxiliadora Aguilera-Romero¹, Joanna Kaminska, Anne Spang, Howard Riezman, Manuel Muñiz

Affiliation

¹ Department of Cell Biology. University of Seville, 41012 Seville, Spain.

Abstract

The p24 family members are transmembrane proteins assembled into heteromeric complexes that continuously cycle between the ER and the Golgi apparatus. These cargo proteins were assumed to play a structural role in COPI budding because of their major presence in mammalian COPI vesicles. However, this putative function has not been proved conclusively so far. Furthermore, deletion of all eight yeast p24 family members does not produce severe transport phenotypes, suggesting that the p24 complex is not essential for COPI function. In this paper we provide direct evidence that the yeast p24 complex plays an active role in retrograde transport from Golgi to ER by facilitating the formation of COPI-coated vesicles. Therefore, our results demonstrate that p24 proteins are important for vesicle formation instead of simply being a passive traveler, supporting the model in which cargo together with a small GTPase of the ARF superfamily and coat subunits act as primer for vesicle formation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ADP-Ribosylation Factor 1 / genetics
ADP-Ribosylation Factor 1 / metabolism
COP-Coated Vesicles / genetics
COP-Coated Vesicles / metabolism*
COP-Coated Vesicles / ultrastructure
Endoplasmic Reticulum / metabolism*
Endoplasmic Reticulum / ultrastructure
Gene Deletion
Golgi Apparatus / genetics
Golgi Apparatus / metabolism*
Golgi Apparatus / ultrastructure
Macromolecular Substances / metabolism
Models, Molecular
Phenotype
Protein Transport / physiology
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae / ultrastructure
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism*
Vesicular Transport Proteins / genetics
Vesicular Transport Proteins / metabolism*

Substances

EMP24 protein, S cerevisiae
ERV25 protein, S cerevisiae
Macromolecular Substances
Saccharomyces cerevisiae Proteins
Vesicular Transport Proteins
ADP-Ribosylation Factor 1