The retinoblastoma protein copurifies with E2F-I, an E1A-regulated inhibitor of the transcription factor E2F

Cell. 1991 Jun 14;65(6):1063-72. doi: 10.1016/0092-8674(91)90558-g.

Abstract

Recently, we identified an inhibitory protein, E2F-I, that blocks the DNA-binding activity of the transcription factor E2F. We also showed that the adenovirus E1A protein reverses this inhibitory activity of E2F-I, thereby restoring the DNA-binding activity of E2F. We have now further purified this inhibitory activity and show that the most purified preparation of E2F-I contains a 105 kd E1A-binding protein. This 105 kd E1A-binding protein cross-reacts with two different antibodies against the retinoblastoma (RB) gene product. Moreover, the RB gene product copurifies with E2F-I activity. Taken together, we conclude that the product of the RB gene is a part of E2F-I and is involved in the regulation of E2F activity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenovirus Early Proteins
  • Animals
  • Base Sequence
  • Carrier Proteins*
  • Cell Cycle Proteins*
  • DNA-Binding Proteins / antagonists & inhibitors*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / isolation & purification
  • E2F Transcription Factors
  • Humans
  • In Vitro Techniques
  • Macromolecular Substances
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Oligonucleotides / chemistry
  • Oncogene Proteins, Viral / genetics
  • Oncogene Proteins, Viral / metabolism
  • Polymerase Chain Reaction
  • Precipitin Tests
  • Retinoblastoma Protein / chemistry
  • Retinoblastoma Protein / isolation & purification
  • Retinoblastoma Protein / metabolism*
  • Retinoblastoma-Binding Protein 1
  • Transcription Factor DP1
  • Transcription Factors / antagonists & inhibitors*
  • Transcription Factors / metabolism*
  • Transcription, Genetic

Substances

  • Adenovirus Early Proteins
  • Arid4a protein, mouse
  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • E2F Transcription Factors
  • Macromolecular Substances
  • Oligonucleotides
  • Oncogene Proteins, Viral
  • Retinoblastoma Protein
  • Retinoblastoma-Binding Protein 1
  • Transcription Factor DP1
  • Transcription Factors