Abstract
Recently, we identified an inhibitory protein, E2F-I, that blocks the DNA-binding activity of the transcription factor E2F. We also showed that the adenovirus E1A protein reverses this inhibitory activity of E2F-I, thereby restoring the DNA-binding activity of E2F. We have now further purified this inhibitory activity and show that the most purified preparation of E2F-I contains a 105 kd E1A-binding protein. This 105 kd E1A-binding protein cross-reacts with two different antibodies against the retinoblastoma (RB) gene product. Moreover, the RB gene product copurifies with E2F-I activity. Taken together, we conclude that the product of the RB gene is a part of E2F-I and is involved in the regulation of E2F activity.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenovirus Early Proteins
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Animals
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Base Sequence
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Carrier Proteins*
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Cell Cycle Proteins*
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DNA-Binding Proteins / antagonists & inhibitors*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / isolation & purification
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E2F Transcription Factors
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Humans
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In Vitro Techniques
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Macromolecular Substances
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Mice
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Molecular Sequence Data
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Molecular Weight
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Oligonucleotides / chemistry
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Oncogene Proteins, Viral / genetics
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Oncogene Proteins, Viral / metabolism
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Polymerase Chain Reaction
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Precipitin Tests
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Retinoblastoma Protein / chemistry
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Retinoblastoma Protein / isolation & purification
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Retinoblastoma Protein / metabolism*
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Retinoblastoma-Binding Protein 1
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Transcription Factor DP1
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Transcription Factors / antagonists & inhibitors*
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Transcription Factors / metabolism*
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Transcription, Genetic
Substances
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Adenovirus Early Proteins
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Arid4a protein, mouse
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Carrier Proteins
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Cell Cycle Proteins
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DNA-Binding Proteins
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E2F Transcription Factors
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Macromolecular Substances
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Oligonucleotides
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Oncogene Proteins, Viral
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Retinoblastoma Protein
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Retinoblastoma-Binding Protein 1
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Transcription Factor DP1
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Transcription Factors