Abstract
We have reported that Erbin expression was down-regulated in the Jurkat leukaemia T lymphocytes treated with the recombinant soluble tumour necrosis factor-related apoptosis-inducing ligand (rsTRAIL). Herein, we studied the expression and the regulation of Erbin and its binding partner, ErbB2, in the MCF-7 breast cancer cell line. We showed that the expressions of Erbin and ErbB2 were modulated by PKCdelta inhibitor, rottlerin, in the TRAIL-resistant MCF-7 cell line. The affinity of Erbin-ErbB2 interaction was reduced by ErbB2 phosphorylation. Inhibiting the expression of Erbin facilitated the sensitivity of the MCF-7 cells to TRAIL via suppressing the ErbB2/AKT/NF-kappaB signalling pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetophenones / pharmacology
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Adaptor Proteins, Signal Transducing / antagonists & inhibitors
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Adaptor Proteins, Signal Transducing / genetics
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Adaptor Proteins, Signal Transducing / pharmacology*
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Apoptosis / physiology
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Benzopyrans / pharmacology
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Blotting, Western
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Breast Neoplasms / metabolism*
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Breast Neoplasms / pathology
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Caspases
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Enzyme Inhibitors / pharmacology
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Female
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Humans
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I-kappa B Kinase / metabolism
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Immunoblotting
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Immunoprecipitation
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NF-kappa B / genetics
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NF-kappa B / metabolism*
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Phosphorylation / drug effects
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Proto-Oncogene Proteins c-akt / metabolism*
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RNA, Small Interfering / pharmacology
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Receptor, ErbB-2 / antagonists & inhibitors
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Receptor, ErbB-2 / genetics
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Receptor, ErbB-2 / metabolism*
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Signal Transduction*
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TNF-Related Apoptosis-Inducing Ligand / pharmacology*
Substances
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Acetophenones
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Adaptor Proteins, Signal Transducing
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Benzopyrans
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ERBIN protein, human
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Enzyme Inhibitors
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NF-kappa B
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RNA, Small Interfering
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TNF-Related Apoptosis-Inducing Ligand
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rottlerin
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Receptor, ErbB-2
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Proto-Oncogene Proteins c-akt
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I-kappa B Kinase
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Caspases