Abstract
The system I cytochrome c biogenesis pathway requires CcmD, a small polypeptide of 69 residues in Escherichia coli. Here it is shown that CcmD is a component of the CcmABC ATP-binding cassette transporter complex. CcmD is not necessary for the CcmC-dependent transfer of heme to CcmE in the periplasm or for interaction of CcmE with CcmABC. CcmD is absolutely required for the release of holo-CcmE from the CcmABCD complex. Evidence is presented that the topology of CcmD in the cytoplasmic membrane is the N terminus outside and the C terminus inside with one transmembrane domain.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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ATP-Binding Cassette Transporters
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Bacterial Proteins / chemistry
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Bacterial Proteins / metabolism
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Bacterial Proteins / physiology*
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Biological Transport
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Cell Membrane
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Cytochromes c / biosynthesis*
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Cytochromes c / chemistry
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Cytochromes c / metabolism*
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Escherichia coli / genetics*
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Escherichia coli / growth & development
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Escherichia coli / metabolism
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Escherichia coli Proteins / chemistry*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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Genes, Bacterial
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Membrane Proteins / chemistry*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Membrane Proteins / physiology*
Substances
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ATP-Binding Cassette Transporters
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Bacterial Proteins
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CcmC protein, bacteria
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CcmD protein, E coli
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Escherichia coli Proteins
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Membrane Proteins
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Cytochromes c