Abstract
The human medulloblastoma cell line DAOY was transfected with Tropomyosin receptor kinase (TrkC), a marker for good prognostic outcome. Following TrkC-activation by its ligand neurotrophin-3, protein extracts from DAOY cells were run on 2DE with subsequent MALDI-TOF-TOF analysis and quantification in order to detect downstream effectors. Protein levels of translational, splicing, processing, chaperone, protein handling, and metabolism machineries were shown to depend on neurotrophin-3-induced TrkC activation probably representing pharmacological targets.
MeSH terms
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Amino Acid Sequence
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Animals
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Biomarkers, Tumor / metabolism
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Cell Line, Tumor
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Child
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Enzyme Activation
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Humans
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Medulloblastoma / chemistry*
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Medulloblastoma / diagnosis
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Medulloblastoma / metabolism*
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Molecular Sequence Data
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Neoplasm Proteins / chemistry*
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism*
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Neurotrophin 3 / metabolism*
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Prognosis
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Receptor, trkC / metabolism*
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Signal Transduction / physiology*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Biomarkers, Tumor
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Neoplasm Proteins
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Neurotrophin 3
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Receptor, trkC