Abstract
We characterized the oxidative stress response of Candida glabrata to better understand the virulence of this fungal pathogen. C. glabrata could withstand higher concentrations of H(2)O(2) than Saccharomyces cerevisiae and even Candida albicans. Stationary-phase cells were extremely resistant to oxidative stress, and this resistance was dependent on the concerted roles of stress-related transcription factors Yap1p, Skn7p, and Msn4p. We showed that growing cells of C. glabrata were able to adapt to high levels of H(2)O(2) and that this adaptive response was dependent on Yap1p and Skn7p and partially on the general stress transcription factors Msn2p and Msn4p. C. glabrata has a single catalase gene, CTA1, which was absolutely required for resistance to H(2)O(2) in vitro. However, in a mouse model of systemic infection, a strain lacking CTA1 showed no effect on virulence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Candida albicans / drug effects
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Candida albicans / genetics
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Candida albicans / growth & development
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Candida glabrata / drug effects
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Candida glabrata / growth & development
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Candida glabrata / metabolism*
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Candida glabrata / pathogenicity*
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Candidiasis / microbiology*
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Catalase / chemistry
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Catalase / genetics
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Catalase / metabolism*
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Gene Expression Regulation, Fungal
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Humans
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Hydrogen Peroxide / pharmacology
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Oxidative Stress*
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Sequence Alignment
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Sequence Deletion
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Virulence
Substances
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Fungal Proteins
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Transcription Factors
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Hydrogen Peroxide
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Catalase