Alzheimer's disease: beta-amyloid precursor protein mRNA expression in mononuclear blood cells

J S Allen; G M Murphy Jr; L F Eng; K E Stultz; H D Davies; L B Pickford; J R Tinklenberg

doi:10.1016/0304-3940(91)90445-y

Alzheimer's disease: beta-amyloid precursor protein mRNA expression in mononuclear blood cells

Neurosci Lett. 1991 Oct 28;132(1):109-12. doi: 10.1016/0304-3940(91)90445-y.

Authors

J S Allen¹, G M Murphy Jr, L F Eng, K E Stultz, H D Davies, L B Pickford, J R Tinklenberg

Affiliation

¹ Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, CA.

PMID: 1838578
DOI: 10.1016/0304-3940(91)90445-y

Abstract

beta-Amyloid precursor protein (beta APP) mRNA was examined in peripheral mononuclear blood cells (PMBCs) in Alzheimer's disease, Down's syndrome and control subjects. Total RNA from PMBCs was reverse transcribed and then amplified using the polymerase chain reaction (PCR). The 3 major beta APP transcripts were expressed in PMBCs from all subjects. These results suggest that PMBCs could be a circulating source for abnormal amyloid deposition in the brain and in peripheral tissues.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Adult
Aged
Aged, 80 and over
Alzheimer Disease / blood
Alzheimer Disease / genetics*
Amyloid beta-Protein Precursor / blood
Amyloid beta-Protein Precursor / genetics*
Down Syndrome / blood
Down Syndrome / genetics
Female
Gene Expression
Humans
Leukocytes, Mononuclear / physiology*
Male
Polymerase Chain Reaction / methods
RNA, Messenger / blood
RNA, Messenger / genetics*
Reference Values

Substances

Amyloid beta-Protein Precursor
RNA, Messenger