Abstract
Objective:
To determine the expression of the genes that code for the isoforms of transforming growth factor-beta (TGF-beta) and TGF-beta receptors (TBR) in mesenchymal stem cells (MSC) from patients with osteoarthritis (OA).
Methods:
Total RNA was extracted from primary cultures of MSC and quantitative real-time reverse transcription-polymerase chain reaction was performed to analyze gene expression.
Results:
MSC from patients with OA showed significantly increased total TGF-beta, TGF-beta1 isoform, TBR-II, and TBR-III mRNA expression compared to controls.
Conclusion:
Our study is the first reporting the gene expression levels of TGF-beta and its isoforms and receptors in patients with OA. These findings might have pathological significance for OA disease.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Aged, 80 and over
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Case-Control Studies
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Cells, Cultured
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Humans
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Mesenchymal Stem Cells / metabolism*
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Mesenchymal Stem Cells / pathology
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Middle Aged
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Osteoarthritis, Knee / metabolism*
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Osteoarthritis, Knee / pathology
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Protein Isoforms / metabolism
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Protein Serine-Threonine Kinases / metabolism
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Proteoglycans / metabolism
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RNA, Messenger / metabolism
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Receptor, Transforming Growth Factor-beta Type I
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Receptor, Transforming Growth Factor-beta Type II
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Receptors, Transforming Growth Factor beta / metabolism*
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Transforming Growth Factor beta / metabolism*
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Transforming Growth Factor beta1 / metabolism
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Transforming Growth Factor beta2 / metabolism
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Transforming Growth Factor beta3 / metabolism
Substances
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Protein Isoforms
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Proteoglycans
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RNA, Messenger
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Receptors, Transforming Growth Factor beta
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Transforming Growth Factor beta
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Transforming Growth Factor beta1
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Transforming Growth Factor beta2
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Transforming Growth Factor beta3
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betaglycan
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Protein Serine-Threonine Kinases
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Receptor, Transforming Growth Factor-beta Type I
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Receptor, Transforming Growth Factor-beta Type II