Abstract
Focusing on CD4(+)CD25(+) regulatory T lymphocytes (T(reg)), we studied the gene expression of T(reg) functional molecules in peripheral blood lymphocytes of patients with paraneoplastic neurological syndrome (PNS), including Lambert-Eaton myasthenic syndrome (LEMS) with small cell lung carcinoma (SCLC) and anti-Hu- or anti-Yo-antibody-positive PNS. T(reg)-rich subsets were sorted from the patients' peripheral blood mononuclear cells, and the mRNA expression levels of their functional genes were measured. The expression levels of FOXP3, TGF-beta and CTLA4 mRNA in T(reg)-rich subsets of PNS patients were down-regulated compared with that of SCLC patients without PNS. These results suggest that T(reg) dysfunction plays a role in PNS development.
MeSH terms
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Adult
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Antibodies / metabolism
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Antigens, CD / genetics
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Antigens, CD / metabolism
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CTLA-4 Antigen
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ELAV Proteins / immunology
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Female
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Flow Cytometry
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Forkhead Transcription Factors / genetics
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Forkhead Transcription Factors / metabolism
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Gene Expression Regulation / physiology
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Humans
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Interleukin-2 Receptor alpha Subunit / metabolism
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Male
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Middle Aged
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Nerve Tissue Proteins / immunology
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Paraneoplastic Syndromes / classification
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Paraneoplastic Syndromes / pathology*
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Paraneoplastic Syndromes / physiopathology*
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RNA, Messenger / metabolism
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T-Lymphocytes, Regulatory / physiology*
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Transforming Growth Factor beta / genetics
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Transforming Growth Factor beta / metabolism
Substances
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Antibodies
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Antigens, CD
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CDR2 protein, human
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CTLA-4 Antigen
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CTLA4 protein, human
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ELAV Proteins
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FOXP3 protein, human
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Forkhead Transcription Factors
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Interleukin-2 Receptor alpha Subunit
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Nerve Tissue Proteins
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RNA, Messenger
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Transforming Growth Factor beta