Comparison between cells and cancer stem-like cells isolated from glioblastoma and astrocytoma on expression of anti-apoptotic and multidrug resistance-associated protein genes

F Jin; L Zhao; H-Y Zhao; S-G Guo; J Feng; X-B Jiang; S-L Zhang; Y-J Wei; R Fu; J-S Zhao

doi:10.1016/j.neuroscience.2008.03.054

Comparison between cells and cancer stem-like cells isolated from glioblastoma and astrocytoma on expression of anti-apoptotic and multidrug resistance-associated protein genes

Neuroscience. 2008 Jun 23;154(2):541-50. doi: 10.1016/j.neuroscience.2008.03.054. Epub 2008 Apr 1.

Authors

F Jin¹, L Zhao, H-Y Zhao, S-G Guo, J Feng, X-B Jiang, S-L Zhang, Y-J Wei, R Fu, J-S Zhao

Affiliation

¹ Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.

PMID: 18462887
DOI: 10.1016/j.neuroscience.2008.03.054

Abstract

This study is to explore and compare the features of the cells and cancer stem-like cells (CSCs) isolated from both glioblastoma and astrocytoma on expression of anti-apoptotic and multidrug resistance-associated protein (MRP) genes. As a result, the mRNA expression of livin, livinalpha and MRP1 was up-regulated in human CSCs from 2 times to 85 times, but the gene expression of MRP3 was down-regulated from 0.09 times to 0.5 times. After just differentiation the mRNA expression of livin, livinalpha and MRP3 was up-regulated from 9 times to 64 times, but the mRNA expression of MRP1 was down-regulated from 0.01 times to 0.03 times. It is a rare report that glioma stem-like cells can be induced successfully from a grade 2-3 astrocytoma tissue. The properties of glioblastoma and astrocytoma stem-like cells on anti-apoptotic and MRP genes are: anti-apoptotic gene livin and survivin are elevated in CSCs but are the most increased in just differentiated CSCs; MRP1 gene is significantly increased and MRP3 is decreased in CSCs, but when differentiating the MRP3 gene starts a remarkable increase in CSCs; the expression of anti-apoptotic and MRP genes shows no differences between the CSCs isolated from glioblastoma and astrocytoma tissues.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

AC133 Antigen
Antigens, CD / metabolism
Apoptosis / physiology
Apoptosis Regulatory Proteins / biosynthesis
Apoptosis Regulatory Proteins / genetics*
Astrocytoma / metabolism*
Astrocytoma / pathology*
Brain Neoplasms / metabolism*
Brain Neoplasms / pathology*
Cell Differentiation / physiology
Cell Line, Tumor
Drug Resistance, Neoplasm / genetics*
Fluorescent Antibody Technique
Glial Fibrillary Acidic Protein / biosynthesis
Glial Fibrillary Acidic Protein / genetics
Glioblastoma / metabolism*
Glioblastoma / pathology*
Glycoproteins / metabolism
Humans
Indicators and Reagents
Intermediate Filament Proteins / biosynthesis
Intermediate Filament Proteins / genetics
Multidrug Resistance-Associated Proteins / biosynthesis
Multidrug Resistance-Associated Proteins / genetics*
Neoplastic Stem Cells / metabolism*
Neoplastic Stem Cells / pathology*
Nerve Tissue Proteins / biosynthesis
Nerve Tissue Proteins / genetics
Nestin
Peptides / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tubulin / biosynthesis
Tubulin / genetics

Substances

AC133 Antigen
Antigens, CD
Apoptosis Regulatory Proteins
Glial Fibrillary Acidic Protein
Glycoproteins
Indicators and Reagents
Intermediate Filament Proteins
Multidrug Resistance-Associated Proteins
NES protein, human
Nerve Tissue Proteins
Nestin
Peptides
Tubulin