Abstract
The N-glycan structures of the Lens culinaris agglutinin (LCA)-reactive fraction of alpha-fetoprotein (AFP-L3), a tumor marker of hepatocellular carcinomas (HCC), were analyzed in relationship to glycosyltransferases and LCA-affinity electrophoresis. Using HPLC and MALDI-TOF MS, we determined the N-glycan structures of AFP from HCC cell lines, and demonstrated they were affected by N-acetylglucosaminyltransferase III and fucosyltransferase VIII, but not by N-acetylglucosaminyltransferase V. Moreover, we identified the N-glycan structures of AFP in HCC patients.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomarkers, Tumor / isolation & purification
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Biomarkers, Tumor / metabolism
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Carbohydrate Sequence
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Carcinoma, Hepatocellular / metabolism
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Carcinoma, Hepatocellular / pathology
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Cell Line, Tumor
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Chromatography, High Pressure Liquid
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Electrophoresis, Agar Gel
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Fucosyltransferases / genetics
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Fucosyltransferases / metabolism
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Glycoproteins / isolation & purification
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Glycoproteins / metabolism*
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Glycosylation
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Glycosyltransferases / genetics
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Glycosyltransferases / metabolism
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Humans
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Liver Neoplasms / metabolism
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Liver Neoplasms / pathology
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Molecular Sequence Data
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N-Acetylglucosaminyltransferases / genetics
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N-Acetylglucosaminyltransferases / metabolism
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Oligosaccharides, Branched-Chain / isolation & purification
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Oligosaccharides, Branched-Chain / metabolism*
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Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / genetics
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Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / metabolism
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Plant Lectins / chemistry
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RNA, Small Interfering / genetics
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Transfection
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alpha-Fetoproteins / isolation & purification
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alpha-Fetoproteins / metabolism*
Substances
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AFP protein, human
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Biomarkers, Tumor
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Glycoproteins
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Oligosaccharides, Branched-Chain
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Plant Lectins
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RNA, Small Interfering
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alpha-Fetoproteins
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lentil lectin
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Glycosyltransferases
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Fucosyltransferases
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N-Acetylglucosaminyltransferases
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beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
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alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase
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Glycoprotein 6-alpha-L-fucosyltransferase
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Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase