The iscS gene deficiency affects the expression of pyrimidine metabolism genes

Hisaaki Mihara; Ryota Hidese; Masahiro Yamane; Tatsuo Kurihara; Nobuyoshi Esaki

doi:10.1016/j.bbrc.2008.05.019

The iscS gene deficiency affects the expression of pyrimidine metabolism genes

Biochem Biophys Res Commun. 2008 Aug 1;372(3):407-11. doi: 10.1016/j.bbrc.2008.05.019. Epub 2008 May 13.

Authors

Hisaaki Mihara¹, Ryota Hidese, Masahiro Yamane, Tatsuo Kurihara, Nobuyoshi Esaki

Affiliation

¹ Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan. mihara@mbc.kuicr.kyoto-u.ac.jp

PMID: 18482579
DOI: 10.1016/j.bbrc.2008.05.019

Abstract

Inactivation of iscS encoding cysteine desulfurase results in a slow growth phenotype associated with the deficiency of iron-sulfur clusters, thiamine, NAD, and tRNA thionucleosides in Escherichia coli. However, the other roles of iscSin vivo are unknown. By using differential screening strategies, we identified 2 pyrimidine salvage enzymes, namely, uridine phosphorylase and cytidine deaminase, which were down-regulated in the iscS mutant. Both enzymes are positively regulated by the cAMP receptor protein (CRP). We also identified a novel protein complex, namely, YeiT-YeiA, whose expression level was decreased in the iscS mutant. The recombinant YeiT-YeiA complex exhibited NADH-dependent dihydropyrimidine dehydrogenase activity, indicating its role in pyrimidine metabolism. The presence of a CRP-binding consensus sequence on the 5'-upstream of the yeiT-YeiA gene suggests its regulation by CRP. These results provide a clue to the possible role of iscS in pyrimidine metabolism by gene regulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Carbon-Sulfur Lyases / genetics
Carbon-Sulfur Lyases / physiology*
Consensus Sequence
Cyclic AMP Receptor Protein / metabolism
Cytidine Deaminase / genetics
Cytidine Deaminase / metabolism
Dihydrouracil Dehydrogenase (NAD+) / genetics
Dihydrouracil Dehydrogenase (NAD+) / metabolism
Dihydrouracil Dehydrogenase (NADP) / metabolism
Down-Regulation
Electrophoresis, Gel, Two-Dimensional
Escherichia coli / genetics*
Escherichia coli / metabolism*
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism
Escherichia coli Proteins / physiology*
Gene Expression Regulation, Bacterial*
Mutation
Pyrimidines / metabolism*
Transcription Factors / metabolism
Uridine Phosphorylase / genetics
Uridine Phosphorylase / metabolism

Substances

Cyclic AMP Receptor Protein
Escherichia coli Proteins
Pyrimidines
Transcription Factors
crp protein, E coli
Dihydrouracil Dehydrogenase (NAD+)
Dihydrouracil Dehydrogenase (NADP)
Uridine Phosphorylase
Cytidine Deaminase
Carbon-Sulfur Lyases
cysteine desulfurase
pyrimidine