Abstract
The autocrine motility factor (AMF) is a multifunctional protein that is involved in tumor progression including enhanced invasiveness via induction of matrix metalloproteinase-3 (MMP3). The increase in MMP3 was found in an AMF-high production tumor cell line, and c-Jun, c-Fos and mitogen-activated protein kinases (MAPKs) were also highly phosphorylated compared with the parent line. AMF stimulation induced the rapid phosphorylation of the cellular MAPK cascade and MMP3 secretion, which was blocked using a specific MAPK inhibitor. Results of this study suggest that AMF stimulation stimulates MMP3 expression via a MAPK signaling pathway.
MeSH terms
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Cell Line, Tumor
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Cell Movement
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Enzyme Activation
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Glucose-6-Phosphate Isomerase / metabolism*
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Glucose-6-Phosphate Isomerase / pharmacology
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Humans
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MAP Kinase Kinase 4 / antagonists & inhibitors
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MAP Kinase Kinase 4 / metabolism*
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Matrix Metalloproteinase 3 / genetics
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Matrix Metalloproteinase 3 / metabolism*
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Mitogen-Activated Protein Kinases / metabolism
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Neoplasm Invasiveness*
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Phosphorylation
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Proto-Oncogene Proteins c-fos / metabolism
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Receptors, Autocrine Motility Factor
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Receptors, Cytokine / genetics
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Receptors, Cytokine / metabolism
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Ubiquitin-Protein Ligases / genetics
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Ubiquitin-Protein Ligases / metabolism
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Up-Regulation
Substances
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Proto-Oncogene Proteins c-fos
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Receptors, Cytokine
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AMFR protein, human
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Receptors, Autocrine Motility Factor
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Ubiquitin-Protein Ligases
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Mitogen-Activated Protein Kinases
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MAP Kinase Kinase 4
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Matrix Metalloproteinase 3
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Glucose-6-Phosphate Isomerase